BIONB 4300 - Experimental Molecular Neurobiology

(PBS-AS)

Spring. 4 credits. Letter grades only.

Prerequisite: BIOMG 3300 or BIOMG 3310. Recommended prerequisite: BIOMG 2801. Enrollment limited to: 16 students.

D. L. Deitcher.

Experiments include PCR, cloning of DNA fragments, RNA purification, restriction digests, bacterial transformation, DNA sequencing, and protein interactions. Experiments emphasize how molecular techniques can be applied to studying neurobiological problems.

Outcome 1: Demonstrate competence in basic molecular biology skills in the laboratory and the underlying principles of these techniques.

Outcome 2: Demonstrate mastery of basic bioinformatics tools such as BLASTN, TBLASTN, and BLASTP and using these tools in analyzing DNA and protein sequences.

Outcome 3: Develop a clear understanding of how different laboratory techniques provide complementary information about gene expression.

Outcome 4: Demonstrate deep understanding of scientific procedures and results through written laboratory reports.